guildfordcycads

Variations in mucous cell parameters in the gills and skin of farmed Atlantic salmon (Salmo salar) from freshwater flow-through and recirculating aquaculture systems compared to the gills of wild Atlantic salmon juveniles

Abstract

This study describes mucous cell (MC) parameters in barrier epithelial tissue of Atlantic salmon (Salmo salar) juveniles reared in freshwater flow-through (FT) and recirculating aquaculture systems (RAS). Using Veribarr™ technology, we found no significant differences in the mean MC size and abundance in the gill lamella and skin between FT and RAS systems (p > 0.05, lme). Notably, 100% of gill lamella MC parameters in RAS facilities fell within the most common range (mean ± 0.5 standard deviation, 38% confidence interval) for farmed salmon gill mucosa, whereas MC parameters in only 20% of FT facilities fell in this most typical range. The differences between individual FT facilities resulted in a greater variability in MC parameters compared to RAS facilities, especially in the gills, likely due to a more controlled environment in RAS. MC sizes and abundances in gills at some FT facilities were comparable to those reported in wild Atlantic salmon juveniles. This finding suggests that the water conditions at these facilities may not evoke a response from the gill mucosa, possibly indicating an environment better suited to the natural physiology of Atlantic salmon. Facility-specific conditions, rather than system-specific conditions, play an important role in the health of salmon barrier tissues. The study underscores the potential of using MC parameters as a monitoring tool, paving the way for innovative baseline health promotion as a preventative disease control strategy in aquaculture systems.

Variations in mucous cell parameters in the gills and skin of farmed Atlantic salmon (Salmo salar) from freshwater flow-through and recirculating aquaculture systems compared to the gills of wild Atlantic salmon juveniles Read More »

Fish oil replacement with linseed oil affects growth, tissue fatty acid composition, blood chemistry, and antioxidant biomarkers in rohu, Labeo rohita

Abstract

The effect of replacing fish oil (FO) with linseed oil (LnO) in rohu, Labeo rohita (initial weight 19.1 ± 0.1 g), diets was evaluated in a 90-day feeding trial. Five isonitrogenous (30% crude protein) and isolipidic (11% crude lipid) diets were prepared by substitution of FO (7% FO in the control) with LnO (0, 25, 50, 75, and 100%). Responses of the fish were tested in triplicate for each diet. Partial replacements up to 75% did not cause any significant change in growth performance and feed efficiency. However, total replacement of FO significantly reduced growth performance and feed utilization (P < 0.05). Survival, hepatosomatic index (HSI), and viscerosomatic index (VSI) as well as whole-body proximate composition were not significantly influenced by the dietary treatments. The ratio of alpha linolenic acid (ALA; 18:3n-3) increased in the muscle and liver with the increasing level of dietary LnO, while the percentage levels of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) decreased. However, EPA and DHA were in considerable level (3.3 and 18.6%, respectively) in muscle even when fed diet with 100% LnO. Hematological parameters such as red blood cell (RBC), hemoglobin (Hb), and hematocrit (Hct) were not influenced by dietary treatments, averaging 2.2 × 106 U/L, 7.6 g/L, and 27.8%, respectively. On the other hand, alanine transaminase (ALT) and aspartate transaminase (AST) activity in serum were significantly elevated with the LnO inclusion levels (P < 0.05), reaching the highest levels (33.0 and 310.7 U/L, respectively) when LnO totally replaced FO. Cholesterol (CHOL), triacylglycerol (TG), high-density lipid (HDL), and low-density lipid (LDL) levels were significantly enhanced (P < 0.05) compared to the control. Cortisol (CORT) and glucose (GLU) were not significantly changed, averaging 1.5 nmol/mL and 20.8 g/dL, respectively. In the liver and serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities were significantly reduced, while malondialdehyde (MDA) level was significantly increased (P < 0.05) with the dietary LnO inclusion. Our results indicate that FO can be substituted with LnO up to 75% in practical diet formulations for rohu without any considerable negative effect, which will substantially decrease the dependency on dietary FO.

Fish oil replacement with linseed oil affects growth, tissue fatty acid composition, blood chemistry, and antioxidant biomarkers in rohu, Labeo rohita Read More »

Seeking input for new forest garden planner


Hey all, I’ve seen that there is a gap with forest garden / food forest digital planners out there, and that most work towards these gardens is still done by hand. I’m working on a new forest garden planner website that I hope will help guide people in designing their forest, geared largely towards beginners like myself. I’ve created a survey (google form) to try to further gauge where people are at and what they’re looking for and would love input from y’all — https://forms.gle/fBghnUZ5BpMpbuPa6 . Thanks!

submitted by /u/Restinor
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Native Plants in 7b


I have a hillside behind my house that doesn’t have water access, but I want to plant some heat tolerant species out there instead of weeds. We live around lots of Sagebrush and grasses.

I’m thinking native grasses and would love to put down some wildflowers/other native flowers. Just wondering if anyone has experience with mass spreading seeds – Yarrow specifically. Where do you buy seeds? Same with grasses? Any help or direction is helpful!

TIA

submitted by /u/bearclaw8458
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My first permacuture project!


https://preview.redd.it/f09uvz4yn5me1.jpg?width=3072&format=pjpg&auto=webp&s=d184889f14172081dcb0d8c23c0bcfca3bf8530a

https://preview.redd.it/1cxrkv4yn5me1.jpg?width=3072&format=pjpg&auto=webp&s=33d7d9ce1902b9a564d076abe6d4e50e511eb512

Hi, I am 14 years old and starting my first permaculture garden next year on a 6800 m² (a bit more than 1½ acres) meadow (just grass). I am looking for some improvement from you. Thanks. When I am a bit older I want to have a sustainable food source, that’s why I am starting with such a young age. In a few years I want to plant on a meadow that’s double the size.

For a bit more insight on my project (the plan is German, but gonna explain):

I plan on planting many old and uncommon varieties. Also I don’t wanna plant too many foreign varieties, instead more cool native varieties. I plan on planting big trees first. Btw the trees on the plan are in real size and have right proportions. I have planned many deadwood piles, sand piles, Kompost piles, stone piles, straw piles, a small pond, two sides of the properties willow fence, bird houses, geese, a flower meadow, a big vegetables garden, many hedges to equalize the loss of diversity due to that I don’t wanna plant the trees close to each other (cause I am poor man), bird trink stations, deadwood hedge, a Berry garden, a tipi, a fire place, a stable and just one big sper through the propertie for the tractor to drive through.

Even tough I planned so many things it still feels like I am missing too much. I think the most important thing is to start, especially planting, since big trees take their time. Next winter I am putting a fence on the right side of the propertie, on the other sides is already a fence. I kinda like having it next to a forest, since it gives a whole other ecosystem for trees who don’t need that much light. I already have many trees and drew a lot of trees. I already gathered many smaller plants for later. I still feel I am not ready, but I kinda know I am. I generally don’t want to rush it. I want to observe, work with the land and be patient. Maybe there is something to add or critic, I would love to read ur comments. Thanks!

submitted by /u/MrBean191
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My first permacuture project! Read More »

What to plant in an acre worth of swales.


So I live here in Mountain Home Idaho and we’re 10-15 years before they plan on putting irrigation in for the county. In the meantime, I’ve made dams in the swales with hay bales to capture most of the watershed. What trees or shrubs would do well in swales that get 12 inches of rain. I have 6 acres total to work with and looking for ideas.

submitted by /u/Beefberries
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What to plant in an acre worth of swales. Read More »

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